Device
Part:BBa_K173010:Experience
Designed by: Lorenzo Pasotti, Susanna Schiavi, Paolo Magni Group: iGEM09_UNIPV-Pavia (2009-10-15)
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how you used this part and how it worked out.
Applications of BBa_K173010
This part was not working in a preliminary induction test.
Materials
- Long term glycerol stocks were stored at -80°C with a final glycerol concentration of 20%
- Antibiotics were: Ampicillin (Amp) 100 ug/ml. All of them were stored at -20°C in 1000x stocks.
- LB medium was prepare with: 1% NaCl, 1% bactotryptone, 0.5% yeast extract. The medium was not buffered with NaOH.
- Ready made IPTG (Sigma) was stored at -20°C in a 200mM stock.
User Reviews
UNIQ9143f08debd439ae-partinfo-00000000-QINU UNIQ9143f08debd439ae-partinfo-00000001-QINU